Journal: Journal of materials chemistry. B

Article Title: Impact of protein corona and light modulation on the antibacterial activity of light-activated silver nanoparticles.

doi: 10.1039/d5tb00081e

Figure Lengend Snippet: Fig. 1 Schematic representation of the experiments carried out in this work. (a) Examine the colloidal stability of PpIX–AgNPs in bacterial culture media; (b) generate the Ag+ release under multi-step irradiation setup in various bacterial culture media; (c) evaluate the bacterial inactivation in MRSA under double-step irradiation condition in NB.

Article Snippet: PpIX–AgNPs were used at a concentration equivalent to 1.5 mg mL 1 in terms of AgNPs or 1 mM in terms of cysPpIX against MRSA (BAA-44 strain from ATCC).

Techniques: Irradiation

Journal: Journal of materials chemistry. B

Article Title: Impact of protein corona and light modulation on the antibacterial activity of light-activated silver nanoparticles.

doi: 10.1039/d5tb00081e

Figure Lengend Snippet: Fig. 2 (a) Schematic representation of PpIX–AgNPs. (b) UV-Vis absorption spectrum for cysPpIX, AgNPs and PpIX–AgNPs. TEM images of (c) AgNPs and (d) PpIX–AgNPs. Colloidal stability of PpIX–AgNPs in DPBS and bacterial culture media, nutrient broth (NB), tryptic soy broth (TSB), and Luria Bertani broth (LB). (e) Hydrodynamic size (red) and polydispersity (blue) change over 24 h for PpIX–AgNPs in DPBS, NB, TSB, and LB. (f) Visual images of PpIX–AgNP in bacterial culture media at 0 and 24 h. (g) Effect of bacterial culture media on z-potential values. (h) UV-Vis absorption spectrum for PpIX–AgNPs in bacterial culture media.

Article Snippet: PpIX–AgNPs were used at a concentration equivalent to 1.5 mg mL 1 in terms of AgNPs or 1 mM in terms of cysPpIX against MRSA (BAA-44 strain from ATCC).

Techniques:

Journal: Journal of materials chemistry. B

Article Title: Impact of protein corona and light modulation on the antibacterial activity of light-activated silver nanoparticles.

doi: 10.1039/d5tb00081e

Figure Lengend Snippet: Fig. 5 Antibacterial activity assessed in MRSA under variable light irradiation and culture conditions. For single irradiation, the timepoint 0 h indicates the inactivation immediately post-irradiation, whereas 4 h and 24 hours correspond times post-irradiation. Similarly, for dual-step, 0 h indicates the inactivation achieved after dual-step irradiation (immediately after 2nd irradiation). The other two time points, 4 and 24 h, show the antibacterial effect post-dual-step irradiation. The error bar is the standard deviation of three independent experimental replicates. (a) The antibacterial activity of 1.5 mg mL1 PpIX–AgNPs in DPBS tested in single (green) and dual-step (red) irradiation setup. (b) The antibacterial activity of varying concentrations of PpIX–AgNP in DPBS tested in a dual-step irradiation setup. (c) The antibacterial activity of varying concentrations of PpIX–AgNP in NB. All the statistical analyses were performed with one-way ANOVA (with Tukey mean comparison) at a = 0.05 and reported as stars assigned to the p-values: ***p r 0.0001, **p r 0.001, *p r 0.05, and ns p 4 0.05.

Article Snippet: PpIX–AgNPs were used at a concentration equivalent to 1.5 mg mL 1 in terms of AgNPs or 1 mM in terms of cysPpIX against MRSA (BAA-44 strain from ATCC).

Techniques: Activity Assay, Irradiation, Standard Deviation, Comparison